Journal: iScience

Article Title: Lens regeneration in situ using hESCs-derived cells —similar to natural lens

doi: 10.1016/j.isci.2023.106921

Figure Lengend Snippet: Regenerated lens expressed specific early lens markers FOXE3 and αA- and αB-crystallin, as well as fiber cell markers β- and γ-crystallin during the regeneration process

Article Snippet: After blocking for 1 hat room temperature with blocking solution (20% bovine serum albumin and 0.5% tritonX-100 [Sigma–Aldrich]), the sections were incubated overnight with the following primary antibodies: mouse anti-FOXE3 (1:200) (Santa Cruz), rabbit anti-β-crystallin (1:200) (Santa Cruz), mouse anti-γ-crystallin (1:200) (Santa Cruz), mouse anti-αB-crystallin (1:100) (Enzo), and mouse anti-αA-crystallin (1:100) (Enzo).

Techniques:

Journal: iScience

Article Title: Lens regeneration in situ using hESCs-derived cells —similar to natural lens

doi: 10.1016/j.isci.2023.106921

Figure Lengend Snippet:

Article Snippet: After blocking for 1 hat room temperature with blocking solution (20% bovine serum albumin and 0.5% tritonX-100 [Sigma–Aldrich]), the sections were incubated overnight with the following primary antibodies: mouse anti-FOXE3 (1:200) (Santa Cruz), rabbit anti-β-crystallin (1:200) (Santa Cruz), mouse anti-γ-crystallin (1:200) (Santa Cruz), mouse anti-αB-crystallin (1:100) (Enzo), and mouse anti-αA-crystallin (1:100) (Enzo).

Techniques: Software